Synergistic potential of Bauhinia holophylla leaf extracts with conventional antifungals in the inhibition of Candida albicans: A new approach for the treatment of oral candidiasis.

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Tác giả: Adriana Araújo de Almeida-Apolonio, Claudia Andréa Lima Cardoso, Thiago Luis Aguayo de Castro, Wellinton Jhon Cupozak-Pinheiro, Deisiany Gomes Ferreira, Cleison da Rocha Leite, Melyssa Negri, Kelly Mari Pires de Oliveira, Stéfani de Oliveira Rosa, João Víctor de Andrade Dos Santos

Ngôn ngữ: eng

Ký hiệu phân loại: 627.12 Rivers and streams

Thông tin xuất bản: England : Archives of oral biology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 216120

OBJECTIVE: This study investigated the combination of Bauhinia holophylla (Bong.) Steud. leaf extracts with conventional antifungal agents, highlighting the extracts' potential as adjuvants in treating oral candidiasis. DESIGN: Ethanolic and aqueous extracts of B. holophylla leaves were analyzed using ultra-high-performance liquid chromatography with a diode array detector (UHPLC-DAD) to assess their chemical composition. Their Minimum Inhibitory Concentration (MIC) against standard strains of Candida albicans and isolates from oral mucosa was determined. Additionally, the potential synergistic effects with chlorhexidine gluconate, nystatin, and fluconazole were investigated, along with their impact on inhibiting and disrupting biofilm formation, germ tube formation of C. albicans, and cytotoxicity in human erythrocytes. RESULTS: Protocatechuic acid, epicatechin, and rutin were identified in both extracts. They exhibited fungistatic activity with a median minimum inhibitory concentration (MIC CONCLUSION: Both ethanolic and aqueous extracts show promising potential as adjuvants in managing oral candidiasis. Notably, the aqueous extract is advantageous due to its non-toxic solvent, cost-effectiveness, and ease of preparation.
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