The widespread development of lignin valorization is hindered by a number of challenges. In particular, efficient valorization necessitates comprehensive characterization of initial lignins. In this work, the structural features of lignins from birch wood (Bétula péndula), obtained by various methods of hard and mild fractionation of biomass: hydrolysis (Hyd-L), kraft (Kraft-L), soda (Soda-L), and soda-ethanol (SodaEt-L) processes, as well as organosolv processes with dioxane (MWL, DL) and dimethyl sulfoxide (DMSO-L) have been comprehensively studied. The main novelty of this study is the first-time application of Two-Dimensional Correlation Infrared Spectroscopy (2D-COS IR) for rapidly and comprehensively assessing structural transformations of lignins, in combination with