Polyhydroxyalkanoates (PHAs) are a group of polymers with a variety of monomers, which are extracted from microorganisms and plants. Due to its good biocompatibility, biodegradability, tunable mechanical property and piezoelectricity, PHAs have been widely used in biomedical fields, such as bone, cartilage, nerve, vascular and skin tissue engineering. This review focuses on the in vivo synthesis, metabolism and biological functions of PHA, and the applications of PHAs in the field of tissue engineering and commercial were also summarized and discussed. Moreover, this review hints the future perspective and research direction of PHA-based materials in the challenging field of tissue engineering. We hope that this review will catalyze the continued advancement and broadening of PHAs' applications in biomedicine.