This cross-sectional study examined the role of mindful parenting in the association between spiritual well-being and positive parenting behaviors among mothers of typically developing children. The sample comprised 381 mothers of children aged 4-17 years. We collected the data using the Three-Factor Spiritual Well-Being Scale (SWBS-3), the Mindfulness in Parenting Questionnaire (MIPQ), and the Multidimensional Assessment of Parenting Scale (MAPS). We tailored our analysis through the subscales of the SWBS-3 (transcendence, harmony with nature, and anomie) to control the contamination effects of other psychological constructs (e.g., meaning and connectedness) on the measurement of spiritual well-being. The correlation analysis yielded significant results, indicating a relationship between the SWBS-3 subscales and mothers' mindful parenting skills (i.e., being in the moment with the child and mindful discipline) and positive parenting practices. Moreover, the mediating effect analysis revealed that mindful parenting skills assumed diverse roles in the relationship between transcendence, harmony with nature, and anomie and positive parenting practices. The findings were then addressed in the context of the existing literature.