Genome-scale spatial mapping of the Hodgkin lymphoma microenvironment identifies tumor cell survival factors.

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Tác giả: Orr Ashenberg, Irving Barrera, Mehdi Borji, Dylan Cable, Daniel Chafamo, Fei Chen, Todd Golub, Saoirse Hanbury, Abner Louissaint, Evan Macosko, Haley Martin, Naeem Nadaf, Gail Newton, Geraldine Pinkus, Scott Rodig, Vignesh Shanmugam, Margaret Shipp, Sean Sullivan, Neriman Tokcan, Caroline Uhler, Jackson Weir

Ngôn ngữ: eng

Ký hiệu phân loại: 616.963 *Diseases due to flukes (Trematode infections)

Thông tin xuất bản: United States : bioRxiv : the preprint server for biology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 218064

A key challenge in cancer research is to identify the secreted factors that contribute to tumor cell survival. Nowhere is this more evident than in Hodgkin lymphoma, where malignant Hodgkin Reed Sternberg (HRS) cells comprise only 1-5% of the tumor mass, the remainder being infiltrating immune cells that presumably are required for the survival of the HRS cells. Until now, there has been no way to characterize the complex Hodgkin lymphoma tumor microenvironment at genome scale. Here, we performed genome-wide transcriptional profiling with spatial and single-cell resolution. We show that the neighborhood surrounding HRS cells forms a distinct niche involving 31 immune and stromal cell types and is enriched in CD4+ T cells, myeloid and follicular dendritic cells, while being depleted of plasma cells. Moreover, we used machine learning to nominate ligand-receptor pairs enriched in the HRS cell niche. Specifically, we identified IL13 as a candidate survival factor. In support of this hypothesis, recombinant IL13 augmented the proliferation of HRS cells
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