Unlocking the potential of Shewanella in metabolic engineering: Current status, challenges, and opportunities.

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Tác giả: Bin Cao, Yidan Hu, Jian Liu, Yi-Nan Liu, Zhourui Liu

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Belgium : Metabolic engineering , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 218909

Shewanella species are facultative anaerobes with distinctive electrochemical properties, making them valuable for applications in energy conversion and environmental bioremediation. Due to their well-characterized electron transfer mechanisms and ease of genetic manipulation, Shewanella spp. have emerged as a promising chassis for metabolic engineering. In this review, we provide a comprehensive overview of the advancements in Shewanella-based metabolic engineering. We begin by discussing the physiological characteristics of Shewanella, with a particular focus on its extracellular electron transfer (EET) capability. Next, we outline the use of Shewanella as a metabolic engineering chassis, presenting a general framework for strain construction based on the Design-Build-Test-Learn (DBTL) cycle and summarizing key advancements in the engineering of Shewanella's metabolic modules. Finally, we offer a perspective on the future development of Shewanella chassis, highlighting the need for deeper mechanistic insights, rational strain design, and interdisciplinary collaboration to drive further progress.
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