Sacbrood virus (SBV) is a significant pathogen affecting honeybee health, leading to substantial economic losses in apiculture. Although traditional methods, like reverse transcription quantitative polymerase chain reaction, offer accurate detection and quantification of SBV, they have limitations for use in field settings, such as apiaries. Here, we developed and evaluated the XQ SBV Dx Kit as a diagnostic tool for the XQ Station point-of-care (POC) RT-qPCR device, which integrates nucleic acid extraction, gene amplification, and detection for on-site molecular diagnosis. Diagnostic performance was assessed using clinical samples infected with SBV and was compared with that of standard laboratory-based RT-qPCR. The limit of detection (LOD) for both methods was 10