Effectors are the most critical weapons that Puccinia striiformis f. sp. tritici (Pst) employs to engage with wheat (Triticum aestivum L.). Discovering important effectors is essential for deciphering the pathogenic mechanisms of Pst. In this study, we identified the expansin-like protein 1from Pst (PstEXLX1), which suppresses cell death in Nicotiana benthamiana. In wheat, knockdown of PstEXLX1 diminished Pst development, whereas PstEXLX1 overexpression enhanced Pst virulence by inhibiting PAMP-triggered immunity (PTI), indicating its importance in pathogenesis. Further investigation revealed that PstEXLX1 stabilizes itself through self-association mediated by its expansin-like domain, which also determines its association with the wheat formate dehydrogenase TaFDH1. Wheat lines overexpressing TaFDH1 exhibited increased resistance to Pst, which was associated with elevated TaFDH1 catalytic activity and induced defense responses. In addition, TaFDH1 activity was strongly inhibited in the presence of PstEXLX1 but became more robust in PstEXLX1-silenced plants, suggesting that PstEXLX1 suppresses TaFDH1 activity. Collectively, our results uncover a strategy employed by Pst to facilitate infection, wherein PstEXLX1 suppresses TaFDH1 activity to repress host immune responses.