Crops are often subjected to various abiotic stresses and interactions between them may occur, but how plants cope with them remains poorly understood. This study explored how combined nitrogen and drought stress impact tomato vascular stem anatomy, nutrient partitioning and amino acids profile. Tomato seedlings were exposed to control (CTR
100N + 100%W), N stress (N
50%N), drought stress (W
50%W), or combined stress (N + W
50%N+50%W) for 27 days. All treatments similarly reduced the phloem and xylem areas. Plants under N + W stress exhibited increased root synthesis of asparagine and arginine (up to 230% compared to W stress and 66% compared to N stress) and showed a higher reallocation and synthesis of osmolytes such as K