As a typical class of environmental endocrine disruptors, imidacloprid (IMI) poses a potential threat to the sustainable survival and reproduction of living beings and human beings. Consequently, it is crucial to establish an efficient degradation method to remove imidacloprid from the environment. The present study aimed to investigate the immobilized laccase for the detoxification of imidacloprid via multi-spectroscopy and molecular docking. Herein, laccase was immobilized on magnetic nanoparticles (NiFe