This study standardized the protein extraction protocol from bee pollen by disrupting the cell wall to obtain bee pollen protein isolates (BPPI) using different pre-treatments: ultrasonication (US-BPPI), pectinase (PE-BPPI), and a combination of ultrasonication and pectinase (US-PEBPPI). The extraction and precipitation pH variables were also optimized, with an extraction pH of 10.5 and precipitation pH of 4.5 identified as ideal based on protein yield and purity. Compared to untreated BPPI, US-PE-BPPI demonstrated significant enhancement in yield (70 %) and purity (20 %) alongside an increase in solubility (88 %), water and oil holding capacity (286 % and 225 %), and emulsifying activity index (223 m