Purification, characterization, structural elucidation, and industrial applications of thermostable alkaline protease produced by seaweed-associated Nocardiopsis dassonvillei strain VCs-4.

• Tác giả: Avani M Ghoghari, Sangeeta D Gohel, Vaishali R Majithiya
• Ngôn ngữ: eng
• Ký hiệu phân loại: 338.9 Economic development and growth
• Thông tin xuất bản: Netherlands : International journal of biological macromolecules , 2025
• Bộ sưu tập: NCBI
• ID: 225218

Tóm tắt

Alkaline proteases are crucial in the enzyme industry. The seaweed-associated strain Nocardiopsis dassonvillei strain VCs-4 produced a protease with the optimal activity of 362.97 U/mL under haloalkaliphilic conditions. Kinetic analysis revealed a Km of 0.17 mg/mL, Vmax of 1928 U/mL/min, and Kcat of 16.66 s