How to maintain high catalytic activity and stability in the process of biocatalysis is crucial, inspiring strategies to construct an appropriate immobilized hydrogel system. Inspired by biologically relevant interactions of natural polymers, we crafted a polysaccharide-based dynamic hydrogel through imine bond among lipase, oxidized dextrans (ODex) and carboxymethylchitosan. The successful preparation of ODex and the in situ immobilization of lipase through a Schiff base reaction were verified by FT-IR,