Transition metals at higher concentrations are toxic to the cells. Membrane bound, ATP-driven efflux pumps belonging to the P-type ATPase superfamily maintain metal homeostasis by transporting metals/ions across the biological membranes. A soil bacterium, Chryseobacterium sp. strain PMSZPI, residing in metal enriched environment of uranium ore deposit exhibited high tolerance to multiple heavy metals. In an attempt to unveil one of the molecular determinants of metal resistance in PMSZPI, we characterized an unannotated, novel metal exporting P