Macrophage phenotypes modulate neoangiogenesis and fibroblast profiles in synovial-like organoid cultures.

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Tác giả: Bruce A Bunnell, Simon Kwoon-Ho Chow, Qi Gao, Stuart B Goodman, Hang Lin, Meagan J Makarcyzk, Masatoshi Murayama, Madison Sidney Virgil Quig, Issei Shinohara, Laurel Elizabeth Wong, Xiurui Zhang

Ngôn ngữ: eng

Ký hiệu phân loại: 636.0885 Animal husbandry

Thông tin xuất bản: England : Osteoarthritis and cartilage , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 225545

OBJECTIVE: To investigate the interaction among the cells thought to be foundational to inflammation, fibrosis, and angiogenesis in the synovial membrane. METHOD: We encapsulated fibroblasts, polarized macrophages, and endothelial cells in a 3D culture system. We used this model to determine the cellular transcriptional profiles, cytokine secretion, and vascular formation associated with different macrophage phenotype conditions. RESULTS: Neo-angiogenesis reached its maximum level at approximately day 21 in the presence of pro-inflammatory macrophages conditions, but was sustained in the presence of anti-inflammatory macrophages. RNA sequencing revealed an influence of macrophage phenotype on gene expression associated with fibrosis and angiogenesis. Furthermore, by including lipopolysaccharides-coated polyethylene particles (lcPE), an inflammatory stimulus replicating wear debris from joint replacements into our system, insights into the local reaction to byproducts of different biomaterials can be ascertained. CONCLUSION: Chronic inflammation and fibrosis of the synovial membrane are often present in osteoarthritis (OA) and post-total joint arthroplasty. Our results suggest that the progression of inflammatory synovial diseases is influenced by macrophage phenotypes.
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