Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs.

 0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Yong Cao, Zihua He, Wenhao Hou, Yuanfan Sun, Yiying Wang, Yingyin Wu, Rui Zhang, Qihao Zhou

Ngôn ngữ: eng

Ký hiệu phân loại: 005.422 Systems programming for specific types of computers, for specific operating systems, for specific user interfaces

Thông tin xuất bản: United States : Molecular cell , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 226433

Current RNA editing techniques are predominantly limited to single-base edits. Here, we introduce selective cleavages and intramolecular stitches of RNA (SCISSOR) for selective cleavage and intramolecular stitches of RNA. Building on the principle that type III CRISPR complex determines target cleavage positions based on gRNA length in 6-nt increments, we hypothesized that engineering gRNAs with bulge loops could circumvent this rule, allowing for flexible RNA excision. Through systematic evaluation of gRNAs with various bulge loops, we established the rules for precise non-6-nt target cleavage and repair. We observed that the complex tolerates 1- or 2-nt bulge loops and accommodates large bulge loops ranging from 6 to 24 nt. Consequently, SCISSOR could accomplish nearly any length of short fragment excision. With its capability to modify open reading frames, we demonstrate the potential of SCISSOR in repairing frameshift mutations and introducing frameshifts to create immunogenic poly-epitopes in human cells. SCISSOR holds promise in RNA therapy and biomedical research.
Tạo bộ sưu tập với mã QR

THƯ VIỆN - TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP.HCM

ĐT: (028) 36225755 | Email: tt.thuvien@hutech.edu.vn

Copyright @2024 THƯ VIỆN HUTECH