OBJECTIVES: To assess cholera-related knowledge, attitudes and practices (KAP) and identify associated factors in a cholera endemic health zone in the Democratic Republic of the Congo (DRC). DESIGN: A community-based cross-sectional study. SETTING: The study was conducted in Kalemie health zone, which is located in the Tanganyika province in eastern DRC. PARTICIPANTS: A total of 455 participants were enrolled in this study using a three-stage random sampling procedure. MAIN OUTCOME MEASURES: Sociodemographic characteristics, cholera and water, sanitation and hygiene (WASH)-related KAP, and their associated factors. RESULTS: The study revealed that the population demonstrated a good knowledge (64%), a positive attitude (73.6%) and good WASH practices (73.8%) regarding cholera. The identification of cholera symptoms, modes of transmission and means of prevention ranged from 79.6% to 94.3%, 38.2% to 41.5% and 32.5% to 56.3%, respectively. It was also noted a high prevalence of tap water use (93.4%) and limited access to water treatment (43.1% to 57.1%), as well as a significant proportion of latrine access (70.8%) with the presence of open defecation (16.3%). In multivariable logistic regression, poor knowledge about cholera was associated with being aged 18 years or younger, having limited education and practising poor WASH habits. No significant factors influenced participants' attitudes. Poor WASH practices were associated with being from households with daily expenditures of less than 1 USD and 5 USD, as well as having poor knowledge about cholera. In addition, believers from Catholic and Muslim backgrounds were less likely to have poor WASH practices. CONCLUSIONS: Our findings indicate that health education initiatives targeting younger demographics should be intensified to enhance awareness of cholera prevention and household water treatment. Furthermore, there is a need for targeted interventions to provide public standpipes and community boreholes, as well as to improve the coverage of covered pit latrines, especially for poorer households.