This Special Issue aims to be a compilation of new results in the areas of differential and difference Equations, covering boundary value problems, systems of differential and difference equations, as well as analytical and numerical methods. The objective is to provide an overview of techniques used in these different areas and to emphasize their applicability to real-life phenomena, by the inclusion of examples. These examples not only clarify the theoretical results presented, but also provide insight on how to apply, for future works, the techniques used.