Haspin kinase binds to a nucleosomal DNA supergroove.

• Tác giả: Andrew S Eneim, Colin R Gliech, Chad W Hicks, Andrew J Holland, Sanim Rahman, Stacy J Vasquez, Cynthia Wolberger, Xiangbin Zhang
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Nature structural & molecular biology , 2025
• Bộ sưu tập: NCBI
• ID: 233531

Tóm tắt

Phosphorylation of histone H3 threonine 3 (H3T3) by Haspin recruits the chromosomal passenger complex to the inner centromere and ensures proper cell cycle progression through mitosis. The mechanism by which Haspin binds to nucleosomes to phosphorylate H3T3 is not known. Here we report cryogenic electron microscopy structures of the human Haspin kinase domain bound to a nucleosome. In contrast with previous structures of histone-modifying enzymes, Haspin solely contacts the nucleosomal DNA, inserting into a supergroove formed by apposing major grooves of two DNA gyres. This binding mode provides a plausible mechanism by which Haspin can bind to nucleosomes in a condensed chromatin environment to phosphorylate H3T3. We identify key basic residues in the Haspin kinase domain that are essential for phosphorylation of nucleosomal histone H3 and binding to mitotic chromatin. Our structural data provide notable insight into a histone-modifying enzyme that binds to nucleosomes solely through DNA contacts.