While the literature provides evidence supporting the effectiveness of evening sprint interval training (SIT), there remains a limited amount of research investigating the effects of morning SIT among soccer athletes. This study examined the effects of morning versus evening SIT during the preparatory phase on physical and physiological performance in collegiate soccer players. Thirty male players volunteered to participate in the study and were randomly divided into morning, evening, or control groups, each group consisting of 10 subjects. The evaluation of physical (countermovement vertical jump [CMVJ], 20-m sprint, Illinois change of direction, and Yo-Yo intermittent recovery test level 1 [Yo-Yo IR1]) and physiological (graded exercise test and Wingate anaerobic power test) performance took place prior to and following the 7-week SIT, both in the morning and evening testing sessions. Both training groups showed significant improvements in physical and physiological performance from pre- to post-training, regardless of testing session timing (p <
0.05). The morning SIT group exhibited greater adaptive changes (p <
0.05) compared to the evening SIT group in CMVJ, 20-m sprint, Yo-Yo IR1, peak and mean power outputs at both the morning and evening testing sessions. These findings suggest that male soccer players can benefit from both morning and evening SIT sessions
however, conducting SIT in the morning could result in greater adaptive changes than evening training. To optimize physical performance adaptations, coaches should schedule SIT sessions in the morning. It is recommended to schedule conditioning workouts in the morning and team practices in the afternoon for optimal adaptations.