An Analysis of Intrinsic Protein Disorder in Antimicrobial Peptides.

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Tác giả: Michael Antonietti, Mak B Djulbegovic, Sydney Granack, Nedym Hadzijahic, William R Herskowitz, Colin K Kim, David J Taylor Gonzalez, Vladimir N Uversky

Ngôn ngữ: eng

Ký hiệu phân loại: 621.3851 Electrical, magnetic, optical, communications, computer engineering; electronics, lighting

Thông tin xuất bản: Netherlands : The protein journal , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 233680

Antibiotic resistance, driven by the rise of pathogens like VRE and MRSA, poses a global health threat, prompting the exploration of antimicrobial peptides (AMPs) as alternatives to traditional antibiotics. AMPs, known for their broad-spectrum activity and structural flexibility, share characteristics with intrinsically disordered proteins, which lack a rigid structure and play diverse roles in cellular processes. This study aims to quantify the intrinsic disorder and liquid-liquid phase separation (LLPS) propensity in AMPs, advancing our understanding of their antimicrobial mechanisms and potential therapeutic applications. To investigate the propensity for intrinsic disorder and LLPS in AMPs, we compared the AMPs to the human proteome. The AMP sequences were retrieved from the AMP database (APD3), while the human proteome was obtained from the UniProt database. We analyzed amino acid composition using the Composition Profiler tool and assessed intrinsic disorder using various predictors, including PONDR® and IUPred, through the Rapid Intrinsic Disorder Analysis Online (RIDAO) platform. For LLPS propensity, we employed FuzDrop, and FuzPred was used to predict context-dependent binding behaviors. Statistical analyses, such as ANOVA and χ
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