Ticks are a medically important group of arthropods, and their control has become a major challenge due to their widespread resistance to synthetic acaricides. Defatted seed meals of plants in the mustard family, commonly generated as byproducts of vegetable oil and biofuel production are known to possess pesticidal properties but their potential to control ticks remain poorly understood. We evaluated the bio-fumigation activity of defatted seed meals of three mustard family species, Brassica juncea (L) Czern. (PG), Lepidium sativum L. (Ls) and Thlaspi arvense L. (DFP), against three medically important hard tick species: Ambylomma americanum (L.) [Aa], Dermacentor variabilis (Say) [Dv] and Ixodes scapularis (Say) [Is]. Volatiles produced by defatted seed meals of the three plant species had strong bio-fumigation activity against ticks, but their effects varied among the three tick species. Toxicity of DFP seed meals was significantly different among the three tick species (no overlap of the 95% CI) with LD