BACKGROUND & OBJECTIVE: Cervical cancer is the second most common malignancy among women worldwide. The Receptor for Activated C Kinase 1 (RACK1) has a key role in regulating the pathways of cell signaling, angiogenesis, cell migration, invasion, and metastasis. This study investigated the association of polymorphisms rs1279736 and rs3756585 with cervical cancer. METHODS: In this study, 100 LB pap-smear and paraffin block samples of cervical squamous carcinoma associated with Human RESULTS: The rs1279736 polymorphism shows that the chance of cervical cancer in individuals with CA and AA genotypes is 3.772 and 1.852 times that of CC genotypes, respectively. This suggests that the A allele is involved in developing cervical cancer ( CONCLUSION: The present study was conducted for the first time. Our results showed that two polymorphisms are significantly associated with cervical cancer.