BACKGROUND: Antimicrobial resistance (AMR) is a growing global health threat, partly driven by inappropriate antibiotic use. Healthcare students, as future practitioners, must have a good understanding of AMR to contribute to antimicrobial stewardship. This study aimed to evaluate the impact of an educational intervention on healthcare students' knowledge of AMR and to assess patterns of antibiotic use prior to the intervention. METHODS: A quasi-experimental pre-and-post study design was conducted with unpaired groups of healthcare students. An educational intervention was carried out comprising of two 20-minute PowerPoint lectures, as well as question and answer sessions focused on understanding the spread, impact, and prevention of AMR. A self-administered questionnaire was given as a pre-test and repeated as a post-test immediately after the training. Students' knowledge of AMR and patterns of antibiotic use was assessed pre-intervention while knowledge of AMR was assessed post-intervention. The knowledge was categorised into domains (Understanding Antibiotic and Antimicrobial Resistance
Spread and Impact of Antibiotic/Antimicrobial Resistance
Prevention of Antibiotic/Antimicrobial Resistance). The total score for each domain of knowledge tested was categorized into good knowledge (≥ 80%) and poor knowledge (<
50%). RESULTS: The number of participants in the pre- and post-intervention were 185 and 157 students with average age of 17.7 years and 17.8 years respectively. Post-intervention, there was a significant increase in students' AMR knowledge, with correct responses regarding spread and impact of AMR increasing from 40.5% pre-intervention to 62.4% post-intervention (p <
0.001
φ (effect size = 0.218). The proportion of students demonstrating good overall knowledge increased from 28.1 to 44.6% (p = 0.001
φ (effect size = 0.171). Misconceptions, such as misunderstanding that antibiotic resistance does not result from the body becoming resistant to antibiotics, persisted but not statistically significant (24.9-17.2%, p = 0.055). Pre-intervention also revealed varied antibiotic use patterns, including the inappropriate use of antibiotics for malaria (51.7%, (n = 76)), and common cold (25.2% (n = 37)). CONCLUSIONS: The study findings indicate that educational interventions can effectively improve healthcare students' knowledge of AMR, though persistent misconceptions underscore the need for more targeted educational efforts. Incorporating structured AMR content into healthcare curricula could help the students contribute to antimicrobial stewardship. Future research should evaluate knowledge retention and behaviour changes to optimize the long-term impact of AMR education.