BACKGROUND: Sepsis requires prompt diagnosis for effective management. Standard Antimicrobial Susceptibility Testing (AST) is time-consuming, highlighting the need for Rapid Antimicrobial Susceptibility Testing (RAST) to provide reports earlier. PURPOSE: To compare the results of RAST and AST in order to determine the feasibility and reliability of RAST. MATERIALS AND METHODS: A study at a tertiary care facility analyzed 95 positive blood culture isolates. RAST was performed by Disk Diffusion (DD) and VITEK 2 Compact Automated Broth Microdilution (BMD) from flagged positive broth. In contrast, AST was performed by DD and BMD from isolated colonies. RESULTS: Among 95 Gram-negative bacteria (GNB), E. coli (n=40), and K. pneumoniae (n=19) were most common. RAST vs AST DD for GNB showed 91% Categorical agreement (CA) with an overall Very Major Error (VME) and Major Error (ME) rates of 0.7% & 2.2% and respectively. For GNB, RAST vs. AST BMD demonstrated a CA of 97% and Essential Agreement (EA) of 97.7%, with VME and ME rates of 1.7% (excluding E.coli+Cefepime drug bug pairs) and 0.1% respectively. CONCLUSION: These findings suggest that RAST is a reliable antimicrobial susceptibility tool for GNB from direct Blood Culture broths. RAST can support early initiation of targeted antimicrobial therapy in patients with sepsis.