INTRODUCTION: The extensive use of pulsed field ablation (PFA) in clinical settings has unveiled new evidence suggesting potential involvement in red blood cell structural impairment resulting in hemolysis and potential acute kidney injury (AKI). METHODS: In this study, blood samples were collected from 16 patients before and 24 h after pulmonary vein isolation (PVI) via a novel investigational PFA-based technology. Biochemical analyses were performed to assess hemolysis and AKI, including total and indirect bilirubin, lactate dehydrogenase (LDH), haptoglobin, plasma free hemoglobin, urea, creatinine, and estimated glomerular filtration rate (eGFR). RESULTS: Based on the periprocedural changes in hemolysis biomarkers, none of the patients displayed evidence of clinically relevant hemolysis following PVI with the PFA catheter.