Immobilization of enzymes on inorganic supports such as silica and carbon materials is an effective approach for chemical surface modification. In this work, hierarchical zeolite (HZ-SAPO's) materials were fabricated by a modified method, and mesoporous carbon (CMK-3) was synthesized using the SBA-15 mesoporous silica as a template. A variety of biocatalysts was prepared using HZ-SAPO with CMK to furnish the nanocomposite biocatalyst. The functionalization of amine group with APTES was done which was further immobilized by Soybean Peroxidase (SBP) enzyme. The material was subjected to a comprehensive characterization process utilizing numerous systematic methods, including X-ray diffraction, N