Since their discovery over a decade ago, MXenes have transformed the field of "materials for healthcare", stimulating growing interest in their healthcare-related applications. These developments have also driven significant advancements in MXenes' synthesis. This review systematically examines the synthesis of MXenes and their applications in sensing and biomedical fields, underscoring their pivotal role in addressing critical challenges in modern healthcare. We describe the experimental synthesis of MXenes by combining appropriate laboratory modules with the mechanistic principles underlying each synthesis step. In addition, we provide extensive details on the experimental parameters, critical considerations, and essential instructions for successful laboratory synthesis. Various healthcare applications including sensing, biomedical imaging, synergistic therapies, regenerative medicine, and wearable devices have been explored. We further highlight the emerging trends of MXenes,