UNLABELLED: Dysbiosis of intratumoral fungal and bacterial communities is associated with poor prognosis in various cancers. However, the mycobiome characteristics in laryngeal squamous cell carcinoma (LSCC) and its correlation with recurrence have not yet been investigated. The mycobiome in 80 LSCC samples was characterized using internal transcribed spacer sequencing, encompassing both tumor tissues and their matched para-cancerous tissues. The intratumoral bacteriome was further identified using 16S rRNA sequencing. These two microbial communities were analyzed using bioinformatics and statistical methods to determine its potential correlation with LSCC recurrence. The fungal alpha diversity in tumors was higher compared with that in para-cancerous tissues ( IMPORTANCE: Our results revealed that dysbiosis of intratumoral microbiota, including increased fungal community diversity and overgrowth of several fungal or bacterial organisms, is substantially linked to the recurrence of LSCC. Drinking habits might alter the laryngeal microbiota to influence the recurrence of LSCC. We also explored a method to potentially predict the recurrence of LSCC from a novel perspective. These findings could offer insights into the etiology of LSCC and pave way to prevent and treat LSCC.