Nghiên cứu tạo khối ung thư gan người bằng dòng tế bào ung thư Hep-3B trên thực nghiệm=Research on creating human liver cancer tumors using Hep-3B cancer cell line in experiments

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Tác giả: Trường Thanh Hưng Lưu, Song Hài Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Y học cộng đồng, 2024

Mô tả vật lý: tr.198-203

Bộ sưu tập: Metadata

ID: 242167

Thêm vào giỏ

Tạo ra khối ung thư gan người trên cơ thể chuột thiếu hụt miễn dịch (chuột nude) bằng dòng tế bào ung thư gan người Hep-3B. Phương pháp nghiên cứu: Nuôi cấy và tăng sinh tế bào ung thư gan người Hep-3B, ghép liều 106 tế bào Hep-3B dưới da mỗi đùi chuột nude, theo dõi tình trạng chuột, sự hình thành và phát triển khối u trong 35 ngày. Xác định tỷ lệ mọc u, tỷ lệ thoái u, thể tích khối u, tỷ lệ sống - chết, hình ảnh giải phẫu bệnh lý của khối u. Kết quả: Đã nuôi cấy và tăng sinh thành công tế bào ung thư gan của người dòng Hep-3B, đủ số lượng tế bào phục vụ ghép tạo khối u trên chuột nude. Khi ghép tế bào ung thư gan người dòng Hep-3B tỷ lệ xuất hiện khối u đạt 87,5 % sau 7 ngày ghép, tỷ lệ thoái u 0 %, thể tích khối u trung bình sau 35 ngày ghép 774,8 mm3, không có chuột nào chết do lỗi kỹ thuật và trong suốt quá trình thí nghiệm, hình ảnh giải phẫu bệnh tương đồng như khối u trên người. Kết luận: Tạo được mô hình chuột thiếu hụt miễn dịch mang khối ung thư gan người dòng Hep-3B.To generate human liver cancer tumors in immunodeficient mice (nude mice) using the human liver cancer cell line Hep-3B. Methods: Cultivation and proliferation of Hep-3B human liver cancer cells, transplantation of 106 Hep-3B cells under the skin of each nude mouse thigh, monitoring mouse condition, formation and development tumor for 35 days. Determine the tumor growth rate, tumor regression rate, tumor volume, survival - death rate, and pathological images of the tumor. Results: Successfully cultured and proliferated human liver cancer cells of the Hep-3B line, enough cells to serve tumor grafting on nude mice. When transplanting human liver cancer cells from the Hep-3B line, the tumor appearance rate reached 87.5% after 7 days of transplantation, the tumor regression rate was 0%, the average tumor volume after 35 days of transplantation was 774.8 mm3. No mice died due to technical errors and throughout the experiment, the pathological images were similar to human tumors. Conclusion: Creating an immune-deficient mouse model carrying human liver cancer of the Hep-3B line.

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