Sự phân bố các chỉ điểm chỉ điểm gen liên quan kháng thuốc của Plasmodium falciparum tại bốn tỉnh Tây Nguyên (2019-2021)=distribution of drug resistance-related genetic markers of Plasmodium falciparum in four provinces of the central highlands (2019-2021)

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Tác giả: Hồng Quang Huỳnh, Thị Hạnh Diệu Lê, Thị Liên Hạnh Nguyễn, Thị Minh Trinh Nguyễn, Thu Hương Nguyễn, Xuân Xã Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Y học cộng đồng, 2024

Mô tả vật lý: tr.79-86

Bộ sưu tập: Metadata

ID: 242204

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Determing the molecular markers related to drug resistance in P. falciparumpopulations in four provinces of the Central Highlands.Research methods: Extracted total DNA was used for Nested-PCR techniques to identify four species of malaria parasites
PCR to capture the K13, Exonuclease, and Pfcrt genes
Sanger sequencing to sequence these gene fragments
and realtime-PCR to identify polymorphisms in the plasmepsin2 and Pfmdr1 genes. Results: The study revealed a high prevalence of the C580Y mutation in P. falciparum, which is associated with artemisinin resistance, along with the E415G mutation in the Exonuclease gene and polymorphisms in plasmepsin2 related to piperaquine resistance. Mutations in the Pfcrtgene, such as K76T and A220S (linked to chloroquine resistance), F145I (related to piperaquine resistance), and polymorphisms in the Pfmdr1 gene (associated with mefloquine resistance) were also identified. Conclusion: The study identified K13 mutations related to artemisinin resistance, the Exonuclease E415G mutation associated with piperaquine resistance, Pfcrt mutations linked to chloroquine resistance, as well as plasmepsin2 and Pfmdr1 polymorphisms related to piperaquine and mefloquine resistance.Xác định các chỉ điểm phân tử liên quan kháng thuốc trên quần thể P. falciparum tại bốn tỉnh Tây Nguyên. Phương pháp nghiên cứu: DNA tổng số sau khi được tách chiết được sử dụng cho các ky thuật Nested-PCR để xác định 4 loài ký sinh trùng sốt rét
PCR để thu nhận các gen K13, Exonuclease và Pfcrt
giải trình tự các đoạn gen này bằng phương pháp Sanger
và sử dụng realtime-PCR để xác định các biến thể gen plasmepsin2 và Pfmdr1. Kết quả: Nghiên cứu phát hiện tỷ lệ cao đột biến C580Y trong P. falciparum liên quan kháng artemisinin, cùng đột biến E415G trên gen Exonuclease và biến thể plasmepsin2 liên quan kháng piperaquin. Các đột biến Pfcrt như K76T, A220S (liên quan đến kháng chloroquine), F145I (liên quan đến kháng piperaquin), và biến thể Pfmdr1 (liên quan đến kháng mefloquine) cũng được ghi nhận. Kết luận: Nghiên cứu đã xác định được các đột biến gen K13 liên quan đến kháng artemisinin, đột biến E415G trên gen Exonuclease liên quan đến kháng piperaquin, các đột biến trên gen Pfcrt liên quan đến kháng chloroquine, cùng với các biến thể plasmepsin2 và Pfmdr1 liên quan đến kháng piperaquin và mefloquine.

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