The 2022 Shanghai COVID-19 lockdown may lead to mental health issues among healthcare workers (HCWs). A cross-sectional study was conducted to investigate psychological health and its associated factors among HCWs. A total of 253 hCWs were recruited in Shanghai, China, through convenience sampling method. Demographic Questionnaire, Generalized Anxiety Disorder-7, Patient Health Questionnaire-9, Insomnia Severity Index, and The Impact of Event Scale-Revised were used to assess demographic information, anxiety, depression, insomnia, and post-traumatic stress disorder (PTSD), respectively. The associated factors of psychological health were identified by binary logistic regression analysis. The prevalence of anxiety, depression, insomnia, and PTSD was 6.3%, 10.3%, 9.9% and 17.8%, respectively. Female HCWs were more likely to report anxiety (