Xác định thành phần loài và tỷ lệ nhiễm ký sinh trùng sốt rét plasmodium spp.. trên muỗi bằng kỹ thuật sinh học phân tử tại Tây Nguyên=identification of species composition and malaria parasite (plasmodium spp.) infection rates in mosquitoes using molecular biology techniques in Highlands

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Văn Nguyên Đỗ, Hồng Quang Huỳnh, Ái Quốc Lê, Thị Hạnh Diệu Lê, Hồng Sang Nguyễn, Thị Liên Hạnh Nguyễn, Thị Minh Trinh Nguyễn, Thu Hương Nguyễn, Xuân Quang Nguyễn, Xuân Xã Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Y học cộng đồng, 2024

Mô tả vật lý: tr.64-71

Bộ sưu tập: Metadata

ID: 243133

Thêm vào giỏ

Determining the species of malaria vector Anopheles and the rate of Plasmodium spp. infection in Anopheles in four Central Highlands provinces by using molecular biology techniques. Methods: Mosquitoes were morphologically classified according to the National Institute of Malariology, Parasitology, and Entomology classification chart (2008). Species identification was performed using PCR, while the presence of Plasmodium spp. within the mosquitoes was detected using ddPCR targeting the 18S rRNA gene region. Results: In Kon Tum, a total of 13 Anopheles species were collected, including two primary malaria vectors (An. Dirus and An. Minimus) and two secondary vectors (An. aconitus and An. maculatus). In Gia Lai, 14 species of Anopheles were collected, comprising one primary vector (An. Minimus) and two secondary vectors. Similarly, 13 species were identified in Đak Lak, including An. Dirus and two secondary vectors. In Dak Nong, 12 species were documented, consisting of two primary vectors and two secondary vectors. The primary vectors were predominantly collected using cattle-baited traps, with additional methods including bed nets, indoor light traps, and cattle light traps. Of the 694 An. Minimus and An. Dirus mosquito samples collected across the four provinces, 6 samples (0.86%) were positive for Plasmodium spp. In Kon Tum, 3 out of 196 samples (2.8%) were positive, while in Gia Lai, 3 out of 190 samples (1.58%) were positive. All positive samples were identified as An. Minimus. Conclusion: The study confirmed the presence of primary malaria vectors (An. Dirus and An. Minimus) and secondary vectors in four Central Highlands provinces. The ddPCR technique successfully detected Plasmodium spp. in a small proportion of mosquito samples, predominantly in An. Minimus. Ongoing surveillance and control of primary vectors are essential to mitigate the risk of malaria transmission in this region, particularly as mosquito species continue to adapt and exhibit changes in behavior.Xác định thành phần loài và tỷ lệ nhiễm KSTSR trong cơ thể muỗi bằng kỹ thuật sinh học phân tử tại bốn tỉnh Tây Nguyên. Phương pháp: Muỗi được định loại bằng hình thái theo Bảng định loại của Viện Sốt rét-KST-CTTW (2008), được xác định loài bằng kỹ thuật PCR và xác định KSTSR trong cơ thể muỗi bằng kỹ thuật ddPCR dựa trên vùng gen 18S rRNA. Kết quả: Số loài Anopheles thu thập tại Kon Tum là 13 loài, bao gồm 2 vector sốt rét chính là An. dirus và An. minimus và hai vector phụ ở vùng đồi núi là An. aconitus và An. maculatus. Tỷ lệ này ở Gia Lai là 14 loài Anopheles, một vector chính là An. minimus và hai vector phụ
tại Đak Lak là 13 loài, gồm một vector chính là An. dirus và hai vector phụ
tại Đak Nông là 12 loài Anopheles, gồm 2 vector chính là và hai vector phụ. Các vector chính được thu thập bằng các phương pháp: soi gia súc, bẫy màn, bẫy đèn trong nhà và bẫy đèn gia súc. 6/694 mẫu muỗi An. minimus và An. dirus tại 4 tỉnh có mặt KSTSR Plasmodium spp., chiếm tỷ lệ là 0,86%. Tại Kon Tum, 3/196 mẫu có nhiễm KSTSR (2,8%). Tại Gia Lai, 3/190 mẫu có nhiễm KSTSR (1,58%). Tất cả các mẫu muỗi có nhiễm KSTSR đều là muỗi An. minimus. Kết luận: Xác định sự có mặt của các vector sốt rét chính (An. dirus và An. minimus) và phụ (An. aconitus và An. maculatus) tại bốn tỉnh Tây Nguyên. Kỹ thuật ddPCR đã phát hiện KSTSR Plasmodium spp. trong một tỷ lệ nhỏ các mẫu muỗi, chủ yếu là ở loài An. minimus. Cần tiếp tục giám sát và kiểm soát các vector chính để giảm thiểu nguy cơ lây truyền sốt rét tại khu vực này, đặc biệt là trong bối cảnh các loài muỗi ngày càng thích nghi và thay đổi hành vi.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR