Xác định cfRNA tiềm năng trong huyết tương bệnh nhân ung thư đại trực tràng=Identification of potential cfRNA in plasma of colorectal cancer patient

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Tác giả: Thị Ngọc Dung Đặng, Ánh Như Nguyễn, Hoàng Thiên Phúc Nguyễn, Tiến Anh Nguyễn, Văn Thiện Chí Nguyễn, Thị Ánh Tuyết Phạm, Lê Sơn Trần, Thị Trang Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Y học Việt Nam (Tổng hội Y học Việt Nam), 2024

Mô tả vật lý: tr.28-34

Bộ sưu tập: Báo, Tạp chí

ID: 243827

Thêm vào giỏ

Identify some cfRNAs (cell free RNA) circulating in plasma and have potential as new biomarkers for colorectal cancer. Subjects and methods: 144 plasma samples including 72 colorectal cancer patients were selected from October 2023 to April 2024 at Tuyen Quang Provincial General Hospital, Hanoi Medical University Hospital and Central Hospital. 108 military personnel and 72 healthy human plasma samples at the Institute of Medical Genetics. The study uses new generation gene sequencing technology to investigate the expression level of circulating free RNA (cfRNA). Normalized data were analyzed using the Mann–Whitney U test to determine statistically significant differences in expression levels of potential cfRNAs between samples from colorectal cancer patients and healthy subjects. Results: Total cfRNA concentrations of 144 study subjects had no statistically significant difference between the two groups (p = 0.165). After gene sequencing of cfRNA samples, 569 cfRNAs were detected showing statistically significant differences between the two groups (p <
0.05). In which cfRNAs coming from genes (MMP9, IL1R2, PGLYRP1, S100A8, H2AC21) have increased expression more than 2 times and cfRNAs coming from genes (CENPQ, HIP1R, ALB, LIMCH, CENPF) have expression decreased more than 2 times in colorectal cancer group compared to normal people. Conclusion: The study has initially identified 10 potential cfRNAs coming from genes (MMP9, IL1R2, PGLYRP1, S100A8, H2AC21, CENPQ, HIP1R, ALB, LIMCH1, CENPF) circulating in plasma and have potential as New molecular markers for colorectal cancer screening.Xác định một số cfRNA(cell free RNA) lưu hành trong huyết tương và có tiềm năng là chỉ dấu sinh học mới cho ung thư Đại trực tràng (UTĐTT). Đối tượng và phương pháp: 144 mẫu huyết tương bao gồm 72 bệnh nhân UTĐTT đã được lựa chọn từ tháng 10/2023 đến tháng 4/2024 tại Bệnh viện Đa khoa tỉnh Tuyên Quang, Bệnh viện Đại học Y Hà Nội và Bệnh viện Trung ương quân đội 108 và 72 mẫu huyết tương người khỏe mạnh tại viện Di Truyền Y Học. Nghiên cứu sử dụng công nghệ giải trình tự gen thế hệ mới để khảo sát mức độ biểu hiện của các RNA tự do lưu hành trong máu (cfRNA). Dữ liệu chuẩn hóa được phân tích bằng test Mann–Whitney U để xác định sự khác biệt có ý nghĩa thống kê về mức biểu hiện của cfRNA tiềm năng giữa các mẫu từ bệnh nhân UTĐTT và đối tượng khỏe mạnh. Kết quả: Nồng độ cfRNA tổng số của 144 đối trượng nghiên cứu không có sự khác biệt có ý nghĩa thông kê giữa hai nhóm (p = 0,165). Sau khi giải trình tự gen mẫu cfRNA, phát hiện 569 cfRNA biểu hiện sự khác biệt có ý nghĩa thống kê giữa hai nhóm (p <
0.05). Trong đó các cfRNA đến từ gen (MMP9, IL1R2, PGLYRP1, S100A8, H2AC21) có biểu hiện tăng hơn 2 lần và các cfRNA đến từ gen (CENPQ, HIP1R, ALB, LIMCH, CENPF) có biểu hiện giảm hơn 2 lần ở nhóm UTĐTT so với người bình thường. Kết luận: Nghiên cứu đã bước đầu xác định được 10 cfRNA tiềm năng đến từ gen (MMP9, IL1R2, PGLYRP1, S100A8, H2AC21, CENPQ, HIP1R, ALB, LIMCH1, CENPF ) lưu hành trong huyết tương và có tiềm năng là các chỉ dấu phân tử mới cho tầm soát UTĐTT.

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