Vai trò của Multiplex Realtime PCR và nuôi cấy trong chẩn đoán nhiễm khuẩn hô hấp cấp ở trẻ em tại Bệnh viện Đa khoa Tâm Anh năm 2023=The role of Multiplex Realtime PCR and culture in diagnosing acute respiratory infections in children at Tam Anh Hospital in 2023

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Huyền My Bùi, Thị Vân Anh Lê, Thị Hà Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Y học Việt Nam (Tổng hội Y học Việt Nam), 2024

Mô tả vật lý: tr.267-271

Bộ sưu tập: Metadata

ID: 243849

Thêm vào giỏ

Acute respiratory infection is an acute bacterial infection in a part or all of the respiratory tract. The disease is common in young children, especially under 5 years old, and is one of the main causes of death in this age group. Detecting the cause early helps differentiate diagnosis and guide appropriate treatment for patients when drug resistance of bacteria is increasing. Cross-sectional descriptive study on 3528 pediatric patients diagnosed with respiratory infections was performed with bacterial culture and Multiplex realtime PCR using Panel RP4 of the Allplex Respiratory panel assays kit (Seegene). Multiplex realtime PCR showed a higher rate of detecting the pathogens (76.7%) than culture (46.8%). Agents commonly detected by bacterial culture are Haemophilus influenzae (20.4%), Moraxella catarrhalis (13.9%), and Streptococcus pneumoniae (9.1%). Multiplex realtime PCR can detect some atypical bacteria that cannot be detected by culture such as Mycoplasma pneumoniae, Chlamydia pneumoniae, Bordetella pertussis, Legionella. The detection rate of multiple agents by PCR was also higher than culture.Nhiễm khuẩn hô hấp cấp là bệnh nhiễm khuẩn cấp tính ở một phần hoặc toàn bộ đường hô hấp. Bệnh thường gặp ở trẻ nhỏ đặc biệt dưới 5 tuổi và là một trong những nguyên nhân chính gây tử vong ở nhóm tuổi này. Xét nghiệm căn nguyên sớm giúp chẩn đoán phân biệt và định hướng điều trị hợp lý trong thời điểm kháng thuốc ở vi khuẩn ngày một gia tăng. Nghiên cứu mô tả cắt ngang trên 3528 bệnh nhi được chẩn đoán lâm sàng nhiễm khuẩn hô hấp có chỉ định nuôi cấy vi khuẩn và Multiplex realtime PCR sử dụng Panel RP4 của bộ kit Allplex Respiratory panel assays (Seegene) cho thấy Multiplex realtime PCR cho tỷ lệ phát hiện căn nguyên cao hơn (76,7%) so với nuôi cấy (46,8%). Các tác nhân được phát hiện bằng nuôi cấy vi khuẩn thông thường là Haemophilus influenzae (20,4%), Moraxella catarrhalis (13,9%) và Streptococcus pneumoniae (9,1%). Multiplex realtime PCR có thể phát hiện được một số vi khuẩn không điển hình hoặc những vi khuẩn khó nuôi cấy như Mycoplasma pneumoniae, Chlamydia pneumoniae, Bordetella parapertussis, Legionella. Tỷ lệ phát hiện đa tác nhân của Multiplex realtime PCR cũng cao hơn so với nuôi cấy.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR