Đặc điểm đột biến gene kháng thuốc Rifampicin và Isoniazide của vi khuẩn lao (Mycobacterium tuberculosis) ở tỉnh Đồng Tháp=Characteristics of mutations in rifampicin and isoniazide resistance genes of Mycobacterium tuberculosis in Dong Thap

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Tác giả: Thị Hương Trúc Đinh, Tấn Khang Đỗ, Thế Long Dương, Thị Loan Dương, Hữu Thành Nguyễn, Đắc Lộc Phạm, Ngọc Dung Trần, Thị Hồng Của Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học (Đại học Cần Thơ), 2024

Mô tả vật lý: tr.106-112

Bộ sưu tập: Metadata

ID: 244352

Thêm vào giỏ

Sự xuất hiện của bệnh lao đa kháng thuốc (MDR-TB) đã gây khó khăn trong kiểm soát bệnh lao, và việc chẩn đoán kịp thời MDR-TB là một thách thức đáng chú ý. Mục tiêu nghiên cứu là xác định đặc điểm phân tử của đột biến gen rpoB, katG, inhA liên quan đến khả năng kháng Rifampicin (RIF) và Isoniazid (INH) ở vi khuẩn lao kháng thuốc được phân lập ở tỉnh Đồng Tháp. Tổng cộng có 29 mẫu vi khuẩn lao kháng thuốc (n=29) đã được ly trích DNA bộ gen bằng kỹ thuật NGS từ đó xác định các đột biến trên gen rpoB, katG và inhA. Kết quả cho thấy đột biến Ser450Leu là phổ biến nhất (58,6%) trên gen rpoB. Ngoài ra, một đột biến mới, Ser254Pro, đã được xác định ở 3,4% số mẫu. Nghiên cứu cũng ghi nhận 7 đột biến khác trên gen rpoB: Gln432Lys, Asp435Tyr, Asp435Val, His445Tyr, His445Leu, Ser450Cys và Leu452Pro. Trên gen katG, hai đột biến đã được ghi nhận: Ser315Thr, với tỷ lệ phổ biến là 82,8% và Arg463Leu, được quan sát thấy ở 96,6% các chủng phân lập. Ngoài ra, gen inhA biểu hiện một đột biến đơn lẻ, Ile194Thr (chiếm 3,4%), có liên quan đến khả năng kháng Isoniazid (INH).The advent of Multidrug-Resistant Tuberculosis (MDR-TB) has significantly complicated the control of tuberculosis, with the prompt diagnosis of MDR-TB presenting a notable challenge. This study aimed to elucidate the molecular characteristics of rpoB, katG, and inhA gene mutations associated with resistance to Rifampicin (RIF) and Isoniazid (INH) in drug-resistant Mycobacterium tuberculosis isolated from Dong Thap province. A total of twenty-nine drug-resistant Mycobacterium tuberculosis isolates (n=29) underwent genomic DNA extraction followed by Next-Generation Sequencing (NGS) technique. This analysis facilitated the identification of mutational patterns within the rpoB, katG, and inhA genes. The results showed that Ser450Leu mutation was the most prevalent, occurring in 58.6% of the rpoB gene sequences. Additionally, a novel mutation, Ser254Pro, was identified in 3.4% of the samples. The study also documented seven other mutations within the rpoB gene: Gln432Lys, Asp435Tyr, Asp435Val, His445Tyr, His445Leu, Ser450Cys, and Leu452Pro. Within the katG gene, two mutations were documented: Ser315Thr, with a prevalence of 82.8%, and Arg463Leu, observed in 96.6% of the isolates. Additionally, the inhA gene exhibited a singular mutation, Ile194Thr (representing 3.4%), which is implicated in conferring resistance to Isoniazid (INH).

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