Khảo sát sự hiện diện và phân bố của nấm rễ cộng sinh (orchid mycorrhizal fungi) trên ba loài lan Dendro (Dendrobium SP.), Hồ điệp (Phalaenopsis SP.) và Kiếm (Cymbidium SP.) trồng tại thành phố Thủ Đức=Survey on the presence and distribution of orchid mycorrhizal fungi in the roots and substrate of 3 orchid species Dendrobium sp., Phalaenopsis sp., and Cymbidium sp., grown in Thu Duc City

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Tác giả: Thị Thùy Dương Phạm, Thị Kim Ngân Phan, Gia Nam Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học (Đại học Cần Thơ), 2024

Mô tả vật lý: tr.131-137

Bộ sưu tập: Metadata

ID: 244396

Thêm vào giỏ

Mối quan hệ giữa nấm cộng sinh vùng rễ và rễ cây đóng vai trò quan trọng trong việc hấp thu nước và chất dinh dưỡng của cây trồng. Nghiên cứu được thực hiện nhằm khảo sát sự hiện diện và phân bố của nấm rễ cộng sinh (OMF) trong rễ và giá thể trồng ba loài lan gồm Dendro, Hồ điệp và Kiếm được trồng trên giá thể dớn trắng, than và gỗ thông tại 10 vườn lan ở thành phố Thủ Đức, thành phố Hồ Chí Minh. Mẫu rễ được nhuộm bằng trypan blue, bào tử trong giá thể được phân lập bằng kỹ thuật sàng ướt ly tâm nổi và nhuộm bằng Melzer + PVLG để quan sát hình thái. Kết quả cho thấy trong rễ và giá thể đều có sự hiện diện của 3 chi nấm gồm Glomus, Acaulospora, Septoglomus, ngoài ra còn ghi nhận được 2 dạng bào tử Dr1 và Dr2 trong rễ và dạng bào tử Ki4 trong giá thể. Glomus là chi chiếm ưu thế trong rễ lan. Trong khi đó, chi Acaulospora hiện diện phổ biến trong giá thể trồng lan với tổng mật số bào tử trung bình là 188 bào tử/g giá thể.The symbiotic relationship between root fungi and plant roots plays an important role in the absorption of water and nutrients of plants. The study was conducted to investigate the presence and distribution of orchid mycorrhizal fungi (OMF) in the roots and growing medium of three species of orchid including Dendrobium sp., Phalaenopsis sp., and Cymbidium sp. grown on sphagnum moss, charcoal, and pine substrates in 10 orchid gardens in Thu Duc city, Ho Chi Minh city. The symbiotic fungi in the root samples were stained with trypan blue, and the spores in the media were isolated by the wet sieving and decanting technique and stained with Melzer + PVLG to observe the spore morphology. The results showed that in the roots and the substrate, there was the presence of 3 fungal genera, including Glomus, Acaulospora, and Septoglomus. In addition, 2 spore types of Dr1, and Dr2 in the roots and Ki4 spores in the growing medium were recorded. Glomus was the predominant genus in orchid roots. Meanwhile, Acaulospora was the most commonly present genus in orchid growing media with an average total spore density of 188 spores/g of growing medium.

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