Phân lập và tuyển chọn vi khuẩn trong nước thải chế biến thủy sản có khả năng hấp thu ammonium=Isolation of ammonium absorbing bacteria in seafood processing wastewater

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Tác giả: Đắc Khoa Nguyễn, Thị Khánh Lam Nguyễn, Thị Phi Oanh Nguyễn, Vũ Luân Trương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học (Đại học Cần Thơ), 2024

Mô tả vật lý: tr.86-96

Bộ sưu tập: Metadata

ID: 244432

Thêm vào giỏ

Nước thải chế biến thủy sản có chứa protein nên khi bị phân hủy tạo amine, ammonia có mùi hôi gây ảnh hưởng đến sức khỏe cộng đồng. Trong nước, ammonia được chuyển thành ammonium và được vi khuẩn hấp thu cho sự tăng trưởng. Nghiên cứu được thực hiện nhằm phân lập vi khuẩn bản địa trong nước thải chế biến thủy sản có khả năng hấp thu ammonium. Từ 4 mẫu nước thải, 24 dòng vi khuẩn có khả năng hấp thu ammonium đã được phân lập. Kết quả khảo sát chứng tỏ dòng WY3.3 có khả năng hấp thu ammonium (200 ppm) hiệu quả nhất, đạt 94,6% ở thời điểm 24 giờ nuôi cấy. Nuôi cấy thông khí và môi trường có pH = 7 là điều kiện tối ưu cho sự hấp thu ammonium của dòng vi khuẩn WY3.3, đạt hiệu suất lần lượt là 91,9 % và 91,7%. Khi môi trường có bổ sung NaCl 1% và 2%, dòng WY3.3 hấp thu ammonium cao, đạt lần lượt là 99,1% và 97%. Phân tích trình tự gen 16S-rRNA cho thấy dòng WY3.3 tương đồng 99,44% với loài Bacillus funiculus nên được định danh là Bacillus sp. WY3.3.Seafood processing wastewater contains many organic compounds. The decomposing of these substances forms amines and ammonia resulting in odors that affect public health. In aquatic environments, ammonia is converted into ammonium which is used by bacteria for growth. This research aimed at the isolation of indigenous bacterial strains from seafood processing wastewater capable of absorbing ammonium. From 4 water samples taken from a seafood processing wastewater treatment system, 24 bacterial strains able to uptake ammonium were isolated. Our data showed that strain WY3.3 was the potential candidate for ammonium (200 ppm) absorption with an efficiency of 94.6% at 24 hours of incubation. Aeration and neutral pH (pH=7) were optimal conditions for ammonium uptake with efficiencies of 91.9% and 91.7%, respectively. The culture medium was supplemented with NaCl at 1% and 2%, strain WY3.3 was still able to absorb 99.1% and 97% ammonium. Based on 16S-rRNA gene sequence analysis, strain WY3.3 showed 99.44% sequence similarity with Bacillus funiculus and was identified as Bacillus sp. WY3.3.

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