Nghiên cứu nâng cao hàm lượng capsaicin từ cao chiết ớt (Capsicum Annuum L.) và ứng dụng trong phòng trừ sâu hại ở làng nghề hoa cảnh tỉnh Bến Tre=Improving capsaicin content from chili extract (Capsicum Annuum L.) and applying it in pest prevention in Ben Tre province's traditional flower-growing villages

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Tác giả: Minh Thuấn Huỳnh, Thị Kim Ngân Nguyễn, Thanh Danh Tống, Công Nương Trần, Thị Câm Tú Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Công thương, 2024

Mô tả vật lý: tr.330-337

Bộ sưu tập: Báo, Tạp chí

ID: 244465

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In this study, chili pepper was initially extracted with ethanol to get an ethanol extract. The extract was then defatted with hexane, followed by being re-extracted with ethyl acetate to obtain a high capsaicin content extract (EtOAc extract). The results indicate that the optimized conditions for high-yield extraction of capsaicin from chili pepper are 96% v/v ethanol solvent with a solid/liquid ratio of 1:40 (g/mL) at 60°C for 20 minutes and extracting once, and the obtained capsaicin content in EtOAc extract is 38%. The insecticidal activity of the formulation containing EtOAc extract illustrated strong contact toxicity against larvae that cause damage to ornamental flowers. Moreover, the antidepressant activity was significantly enhanced by adding azadirachtin. In field experiments, the insecticide activity of the product was over 85% after 7 days, and no adverse effect was observed on the growth of flowers during the experiments.Trong nghiên cứu này, ớt được chiết bằng dung môi ethanol để thu được cao cồn, sau đó, cao được loại béo bằng hexane và chiết lại bằng ethyl acetate để thu được cao chiết có hàm lượng capsaicin cao. Kết quả nghiên cứu đã xác định được điều kiện tối ưu để cao chiết ớt có hàm lượng capsaicin cao nhất là dung môi EtOH 96% v/v với tỉ lệ rắn lỏng là 1:40 (g/mL), ở 60°C trong 20 phút và chiết 1 lần. Sau khi loại béo bằng hexane và chiết lại bằng ethyl acetate, hàm lượng capsaicin thu được trong cao EtOAc là 38%. Ngoài ra, kết quả khảo sát hoạt tính kháng côn trùng cho thấy cao chiết ớt giàu capsaicin có độc tính tiếp xúc mạnh đối với sâu gây hại hoa cảnh và có khả năng gây ngán ăn mạnh khi bổ sung thêm azadirachtin. Bên cạnh đó, hiệu lực trừ sâu khi thử nghiệm trên vườn hoa đạt trên 85% sau khi phun 7 ngày và việc sử dụng chế phẩm thuốc trừ sâu sinh học không ảnh hưởng đến quá trình sinh trưởng của cây.

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