Tác dụng hạ huyết áp và chống oxy hóa của Nano Rutin trên mô hình tăng huyết áp do N(G)-nitro-L-arginine-methyl ester (L-NAME) trên chuột cống trắng=Blood pressure reducing and antioxidant effects of Nano Rutin on Wistar rats with hypertension model caused by N(G)-nitro-L-arginine-methyl ester (L-NAME)

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Tác giả: Hoàng Ngân Nguyễn, Hồng Hạnh Nguyễn, Thị Thanh Tú Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Nghiên cứu y học (Đại học Y Hà Nội), 2024

Mô tả vật lý: tr.164-172

Bộ sưu tập: Metadata

ID: 244650

Thêm vào giỏ

Nghiên cứu được tiến hành để đánh giá tác dụng hạ huyết áp và chống oxy hóa của Nano Rutin trên mô hình tăng huyết áp do L-NAME (N(G)-nitro-L-arginine-methyl ester) trên chuột cống trắng. Chuột cống trắng chủng Wistar được chia thành 5 lô, mỗi lô 12 con. Lô 1 chuột không gây tăng huyết áp. Với 4 lô còn lại chuột được gây tăng huyết áp bằng cách cho uống L-NAME (0,5% w/v trong nước cất) với liều 50 mg/kg/ngày trong 4 tuần liên tục. Trong đó, lô 2 chuột uống nước cất
lô 3 chuột được uống losartan 25 mg/kg/ngày
lô 4 chuột được uống Nano Rutin liều 280 mg/kg/ngày
lô 5 chuột được uống Nano Rutin liều 560 mg/kg/ngày. Thời gian uống thuốc thử là 4 tuần. Chuột được đo huyết áp tâm thu, huyết áp tâm trương và nhịp tim tại thời điểm trước dùng thuốc, và sau dùng thuốc 1 tuần, 2 tuần, 3 tuần và 4 tuần. Nồng độ NO và các chỉ số đánh giá khả năng chống oxy hoá (SOD, GSH, MDA) trong huyết tương được đo tại thời điểm trước và sau 4 tuần. Kết quả cho thấy, Nano Rutin liều 280 mg/kg và 560 mg/kg có tác dụng ngăn ngừa chứng tăng huyết áp do L-NAME gây ra ở chuột cống trắng và không ảnh hưởng đến nhịp tim của chuột. Ngoài ra, Nano Rutin làm tăng NO, SOD, GSH và giảm MDA trong huyết tương của chuột.This study aimed to evaluate the blood pressure reducing and antioxidant effects of Nano Rutin on Wistar rats with hypertension model caused by N(G)-nitro-L-arginine-methyl ester (L-NAME). The Wistar rats were randomly allocated into 5 groups with 12 animals in each group. Group 1 was nonhypertensive rats. With the remaining 4 groups, the rats were induced to become hpertensive by orally administrating L-NAME (0.5% w/v diluted in distilled water), at 50 mg/kg b.w./day
group 2 was administered with distilled water
group 3 was administered with Losartan at 25mg/kg b.w./day
group 4 was administered with Nano Rutin at 280 mg/kg b.w./day and group 5 was administered with Nano Rutin at 560 mg/kg b.w./day. The duration to take the reagents was 4 weeks. The systolic, the diastolic pressure and the heart rate were assessed at baseline, after 1 week, 2 weeks, 3 weeks and 4 weeks of treatment. The serum levels of nitric oxide (NO) and compounds determining the anti-oxidant capacity such as Superoxide dismutase (SOD), glutathione (GSH), malondylaldehyde (MDA) were assessed at baseline and after 4 weeks of treatment. The results showed that Nano Rutin at the doses of 280 mg/kg b.w. and 560 mg/kg b.w. was effective in preventing L-NAME-induced hypertension in Wistar rats and did not alter the rats’ heart rates. In addition, Nano Rutin increased the serum NO level and the antioxidant capacity in rats administered L-NAME (increased SOD, GSH and decreased MDA).

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