Độc tính cấp và bán trường diễn của viên nang cứng từ bài thuốc “Dạ dày HĐ” trên thực nghiệm=Acute and sub-chronic toxicity of capsule extracted from Da day HD herbal formula in experimental animals

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Thanh Hường Nghiêm, Thị Thanh Tú Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Nghiên cứu y học (Đại học Y Hà Nội), 2024

Mô tả vật lý: tr.173-182

Bộ sưu tập: Metadata

ID: 244651

Thêm vào giỏ

Nghiên cứu nhằm đánh giá độc tính cấp và bán trường diễn của viên nang cứng từ bài thuốc “Dạ dày HĐ” trên thực nghiệm. Nghiên cứu độc tính cấp của viên nang trên chuột nhắt bằng phương pháp Litchfield – Wilcoxon. Nghiên cứu độc tính bán trường diễn của viên nang trên chuột cống trắng theo hướng dẫn của WHO. Kết quả nghiên cứu cho thấy với liều cao nhất (liều 45,0 g/kg thể trọng chuột - gấp 51 lần liều dự kiến dùng trên lâm sàng) không xác định được liều gây chết 50% số động vật thí nghiệm (LD50) của viên nang cứng. Viên nang cứng từ bài thuốc “Dạ dày HĐ” không gây độc tính bán trường diễn trên chuột cống trắng với liều tương đương lâm sàng (0,48 g/kg thể trọng chuột/ngày) và liều gấp 3 liều lâm sàng (1,44 g/kg thể trọng chuột/ngày) trong 12 tuần liên tục uống thuốc. Tất cả các chỉ số theo dõi về tình trạng chung, cân nặng, chức năng tạo máu, chức năng gan, chức năng thận đều nằm trong giới hạn bình thường, mô bệnh học gan, thận không có sự khác biệt rõ rệt so với lô chứng (p >
0,05).This study aimed to evaluate the acute and sub-chronic toxicity of capsule extracted from Da Day HD herbal formular in the experimental animals. The acute toxicity was performed based on the Litchfield - Wilcoxon method. The sub-chronic toxicity was evaluated according to the World Health Organization's guidance. The results showed that the highest dose of capsule extracted from Da Day HD herbal formular (45.0 g/kg b.w./day, 51 times as high as the maximal recommended human dose) did not show acute oral toxicity in mice and the median lethal dose (LD50) was not determined. Administration of capsule extracted from Da Day HD herbal formular at 0.48 g/kg b.w./day (equivalent to clinical dose) and 1.44 g/kg body weight (three times as high as the clinical dose) in 12 consecutive weeks did not produce any significant detrimental effect on the body weight, hematological and biochemical parameters, as well as the gross and microscopic images of the rats’ liver and kidney, compared to control group (p >
0.05).

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