Nghiên cứu độc tính cấp và bán trường diễn của dung dịch uống EATWELLB trên động vật thực nghiệm=Evaluation of acute and subchronic toxicity of EATWELLB in experimental animals

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Tác giả: Thị Thanh Loan Nguyễn, Thanh Tùng Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Nghiên cứu y học (Đại học Y Hà Nội), 2024

Mô tả vật lý: tr.239-249

Bộ sưu tập: Metadata

ID: 244680

Thêm vào giỏ

Nghiên cứu được thực hiện nhằm đánh giá độc tính cấp và bán trường diễn theo đường uống của dung dịch EATWELLB trên động vật thực nghiệm. Nghiên cứu độc tính cấp được tiến hành trên chuột nhắt trắng chủng Swiss theo đường uống và xác định liều gây chết 50% chuột (lethal dose, LD50) theo phương pháp Litchfied-Wilcoxon. Nghiên cứu độc tính bán trường diễn tiến hành theo hướng dẫn của Tổ chức Y tế Thế giới. Chuột cống trắng chủng Wistar được uống EATWELLB liều 1,8 mL/kg và 5,4 mL/kg trong 4 tuần liên tục. Kết quả nghiên cứu độc tính cấp cho thấy EATWELLB liều 75mL dung dịch gốc/kg không gây biểu hiện độc tính cấp. Như vậy, liều dung nạp tối đa của EATWELLB cao gấp 20,8 lần liều dùng dự kiến trên người không gây độc tính cấp. Chưa xác định được LD50 trên chuột nhắt trắng của dung dịch EATWELLB theo đường uống. Kết quả nghiên cứu độc tính bán trường diễn cho thấy tình trạng chung, các chỉ số huyết học, chức năng gan, thận và hình thái vi thể gan, thận không có sự khác biệt so với lô chứng sinh học và so sánh với thời điểm trước khi dùng thuốc thử. Như vậy, dung dịch EATWELLB liều 1,8 mL/kg và 5,4 mL/kg uống trong 4 tuần liên tục không gây độc tính bán trường diễn trên động vật thực nghiệm.The present study investigated the oral acute and subchronic toxicities of the EATWELLB solution in experimental animals. The acute toxicity was conducted using Swiss mice through oral administration, and LD50 was determined by the Litchfield-Wilcoxon method. The subchronic toxicity was studied based on the guidance of the World Health Organization using Wistar rats. EATWELLB was administered orally at 1.8 mL/kg per day and 5.4 mL/kg per day over four weeks. Regarding acute toxicity, EATWELLB did not express acute toxicity in mice at the highest dose used (75 ml/kg). Our findings showed that the maximum tolerated dose was 20.8 times higher than the expected human dose without symptoms of acute toxicity. LD50 of EATWELLB could not be determined in mice. In terms of the subchronic toxicity, EATWELLB caused no significant change in general status, haematological examination, renal and hepatic function tests
in addition, it did not cause any change in the histology of the liver and kidney of experimental rats. Based on aforementionned results, we suggest that EATWELLB at 1.8 mL/kg per day and 5.4 mL/kg per day did not cause subchronic toxicity in experimental animals.

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