Nghiên cứu tính kích ứng da và tác dụng giảm đau của Xịt Cốt thống Tuệ Tĩnh trên động vật thực nghiệm=Study on skin irritation and analgesic effects of Cot Thong Tue Tinh spray on experimental animals

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Thị Hảo Bùi, Thị Minh Thu Nguyễn, Quốc Sự Phạm, Thị Vân Anh Phạm, Trường Vũ Phan

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Y dược học cổ truyền Việt Nam, 2024

Mô tả vật lý: tr.45-51

Bộ sưu tập: Metadata

ID: 245108

Thêm vào giỏ

Đánh giá tính kích ứng da thỏ vờ tác dụng giảm đau của Xịt Cốt thống Tuệ Tĩnh trên chuột nhắt trắng. Đôi tượng và phương pháp: Thử kích ứng da thỏ theo hưởng dẫn của Bộ Y tế vờ OECD. Thử tác dụng giảm đau bằng phương pháp dùng nhiệt kích thích vờ châm kim. Ở m ỗi th ử nghiệm, chuột được chia thành 4 lô, m ỗi lô 10 con, trong đó 1 lô chứng âm được x ịt nước cất, 1 lô chứng dương x ịt Salonpas Jet Spray vờ 2 lô còn lạ i dùng X ịt Cốt thống Tuệ Tĩnh. Kết quà: với liều 1,0 m l bôi trên da thỏ diện tích 2,5 cm X 2,5 cm, m ẫu th ử không gây viêm, ban đỏ hay ph ù nê da thỏ. với liều 0,36 ml, m ẫu thử có tác dụng kéo dài thời gian phàn ứng với đau do nhiệt (p <
0,001), làm tăng có ý nghĩa thống kê lực gây đau và thời gian phản ứng với đau do châm kim so với trước khi dùng thuốc (p<
0,05và p<
0,01). Kết luận: Xịt cốt thống Tuệ Tình và hỗn hợp Xịt cốt thống-ethanol 70P (1:1) không gây kích úng da thỏ, đồng thời có cả tác dụng giám đau trung ương vờ giảm đau ngoại vi ờ chuột nhắt trắng với liều 0,36 ml/ngày.To assess whether Cot Thong Tue Tinh spray causes rabbits' skin irrita tion and has analgesic effect on mice. Subject and methods: Skin irritation testing on rabbits was conducted following the guidelines o f the Ministry o f Health and the OECD. The analgesic effect was evaluated by thermal stimulation (hot plate) and needle prickling methods. In each experiment mice were divided into 4 groups, 10 animals each, o f which the negative control group was sprayed with distilled water, the positive control group was sprayed with SaI on pas Jet Spray and the remaining 2 groups were given Cot Thong Tue Tinh spray. Results: With a dose o f 1.0 m l applied to rabbit skin in an area o f 2.5 cm X 2.5 cm, the test sample did not cause inflammation, erythema, o r edema. Additionally, a t a dose o f 0.36 ml, the test sample had effects including prolonging heat-pain response time (p <
0.001), statistically significant increase in pain force and pain reaction time due to needling compared to those before applying the test samples (p<
0.05 and p<
0.01). Conclusions: Cot Thong Tue Tinh spray as well as its combination with ethanol 70° (1:1) did not irritate rabbits'skin and had both central and peripheral analgesic effects in mice at a dose of 0.36 ml/day.

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