Microbes play an important role in food chain by metabolizing organic matter, cycling nutrients, and maintaining a dynamic equilibrium among organisms in water and sediment. The objective is to study fluctuating taxonomic microbial diversity profile in sediment-water interface at different days of culture (DOC) of Penaeus vannamei in varying salinities using Illumina MiSeq platform. Sediment samples were collected in Tamil Nadu State in India from low saline, brackish water, and high saline ponds on 30, 60, and 90 DOC. Bacterial richness and diversity in species was high in low-saline ponds. Beta diversity variation indicated more differences in bacterial composition in high and low saline ponds. The predominant phyla observed were Proteobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, Firmicutes and Cyanobacteria. High saline ponds accounted for more nitrification bacterial communities, sulfur reducing bacterial communities, sulfur oxidizing bacterial communities and high redox potential, whereas denitrification bacterial communities were high in brackishwater ponds.