SABATH is one class of enzyme belonging to the class ofmethyltransferase, playing a crucial role in plant defense and stress responsemechanisms. Despite its importance, no systematic analysis of the expansion of theSABATH gene family in cassava (Manihot esculenta) has been reported up to date.In this study, we investigated the SABATH gene family in cassava, revealing theirrandom distribution across 18 chromosomes, with variable numbers of gene copiesper chromosome. According to gene duplication analysis, five duplication eventswere identified, primarily segmental duplications, indicating their evolutionarysignificance. The Ka/Ks ratio analysis indicated that most duplicated genes areunder negative selection, preserving their functions, while one pair showed signs ofpositive selection, suggesting adaptive benefits. Gene structure analysis showeddiverse exon counts, primarily three or four. Expression profiling across 11 cassavatissues demonstrated tissue-specific expression patterns, with some genes highly orexclusively expressed in specific tissues such as root apical meristems,embryogenic calli, and fibrous roots, implying distinct functional roles in cassavagrowth and development. Overall, this study provides valuable insights into theevolution and functional diversity of the SABATH gene family in cassava andidentifies candidate genes for further functional characterization.SABATH is one class of enzyme belonging to the class ofmethyltransferase, playing a crucial role in plant defense and stress responsemechanisms. Despite its importance, no systematic analysis of the expansion of theSABATH gene family in cassava (Manihot esculenta) has been reported up to date.In this study, we investigated the SABATH gene family in cassava, revealing theirrandom distribution across 18 chromosomes, with variable numbers of gene copiesper chromosome. According to gene duplication analysis, five duplication eventswere identified, primarily segmental duplications, indicating their evolutionarysignificance. The Ka/Ks ratio analysis indicated that most duplicated genes areunder negative selection, preserving their functions, while one pair showed signs ofpositive selection, suggesting adaptive benefits. Gene structure analysis showeddiverse exon counts, primarily three or four. Expression profiling across 11 cassavatissues demonstrated tissue-specific expression patterns, with some genes highly orexclusively expressed in specific tissues such as root apical meristems,embryogenic calli, and fibrous roots, implying distinct functional roles in cassavagrowth and development. Overall, this study provides valuable insights into theevolution and functional diversity of the SABATH gene family in cassava andidentifies candidate genes for further functional characterization.