This study aims to determine the various strains of yeast and filamentousfungi in traditional alcohol yeast cake from Hai Hau district, Nam Dinh province.By traditional laboratory techniques such as isolation on YPD agar, observation ofcolony morphology as well as cell characteristics by microscopy, combined withmolecular biology method to sequence ITS region 03 strains of yeastSaccharomyces cerevisiae CN1, CN2, and NM3 were identified: 01 pseudo-strainyeast Saccharomycopsis fibuligera NM02 and 01 strain of filamentous fungiRhizopus microsporus NS04. The density of yeast strains cultured on YPD agarsupplemented with 50 mg/L Tetracycline was 4.5 × 108 CFU/g, while the densityof R. microsporus NS04 filamentous fungi was 2.0 × 106 CFU/g. With the agarplate diffusion method, yeast strains of S. cerevisiae CN1, CN2, and NM3 that areunable to biosynthesize extracellular enzymes have been identified. Yeast pseudostrain S. fibuligera NM02 is capable of producing amylase (with starch substratehydrolysis ring diameter >
9 mm), cellulase (16 mm), and protease (18 mm). Thefilamentous fungi R. microsporus NS04 has a higher ability to produceextracellular enzymes such as amylase (>
9 mm), cellulase (33 mm), and protease(39 mm).This study aims to determine the various strains of yeast and filamentousfungi in traditional alcohol yeast cake from Hai Hau district, Nam Dinh province.By traditional laboratory techniques such as isolation on YPD agar, observation ofcolony morphology as well as cell characteristics by microscopy, combined withmolecular biology method to sequence ITS region 03 strains of yeastSaccharomyces cerevisiae CN1, CN2, and NM3 were identified: 01 pseudo-strainyeast Saccharomycopsis fibuligera NM02 and 01 strain of filamentous fungiRhizopus microsporus NS04. The density of yeast strains cultured on YPD agarsupplemented with 50 mg/L Tetracycline was 4.5 × 108 CFU/g, while the densityof R. microsporus NS04 filamentous fungi was 2.0 × 106 CFU/g. With the agarplate diffusion method, yeast strains of S. cerevisiae CN1, CN2, and NM3 that areunable to biosynthesize extracellular enzymes have been identified. Yeast pseudostrain S. fibuligera NM02 is capable of producing amylase (with starch substratehydrolysis ring diameter >
9 mm), cellulase (16 mm), and protease (18 mm). Thefilamentous fungi R. microsporus NS04 has a higher ability to produceextracellular enzymes such as amylase (>
9 mm), cellulase (33 mm), and protease(39 mm).