Thiết lập quy trình khử hóa tế bào kết hợp khử khoáng xương bò để chế tạo mô xương ghép ứng dụng trong nha khoa tái tạo=Establishment of a decellularization combined demineralization process to fabricate bovine-derived xenografts for regenerative dentistry

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Tác giả: Duy Thảo Huỳnh, Khánh Hòa Nguyễn, Thị Thủy Tiên Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2024

Mô tả vật lý: tr.142

Bộ sưu tập: Metadata

ID: 249363

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Elimination of the immune response elicited by grafting remains a challenge for the fabrication of non-autologous bone grafts. The aim of this study was to assess the effectiveness of a decellularization-combined demineralization process to produce bovine-derived demineralized bone matrix (BDMBM) for regenerative dentistry. Bone tissues were harvested from bovine femoral heads, cleaned, and disinfected. The tissues were then decellularized by sequentially soaking in 1 M NaCl, a mixture of 0.075 M KCl and 0.1 % EDTA (1:1, v/v), and a mixture of 0.5 % SDS and 0.1 % EDTA (1:1, v/v). They were further demineralized using a mixture of 8 % HCl and 8 % formic acid (1:1, v/v). The energy-dispersive X-ray spectroscopy found that the contents of calcium and phosphorus in the bone tissues after treatment were significantly reduced by nearly 90 % compared to before treatment (p <
0.001). The dry weight of bone tissues also showed a significant weight loss of up to 30.29 % at the end of process compared to the begining (p <
0.001). The analysis of histological morphology and structural topography under scanning electron microscopy indicated that BDMBM contained no cellular components while maintaining the structure of the extracellular matrix. In conclusion, the decellularization-combined demineralization process used in this study may offer promising method to fabricate BDMBM for bone regeneration applications in dentistry.Mục tiêu nghiên cứu là đánh giá hiệu quả của quy trình khử hóa tế bào kết hợp khử khoáng xương bò để chế tạo mô xương ghép ứng dụng trong nha khoa tái tạo. Mô xương được thu nhận từ chỏm xương đùi bò, làm sạch và khử trùng. Sau đó mô xương được khử hóa tế bào bằng cách ngâm lần lượt trong NaCl 1 M, hỗn hợp KCl 0,075 M và EDTA 0,1 %, hỗn hợp SDS 0,5 % và EDTA 0,1 %. Mô xương tiếp tục được khử khoáng bằng hỗn hợp HCl 8 % và axit formic 8 %. Phổ tán xạ năng lượng tia X ghi nhận hàm lượng canxi và phốt pho trong mô xương giảm gần 90 % so với ban đầu (p <
0,001). Trọng lượng khô của mô xương sau xử lý cũng bị chiết rút rõ rệt lên đến 30,29 % so với trước xử lý (p <
0,001). Hình ảnh mô học và hình chụp dưới kính hiển vi điện tử quét cho thấy mô xương không chứa thành phần tế bào mà vẫn duy trì hình thái cấu trúc của khung ngoại bào. Như vậy quy trình khử hóa tế bào kết hợp khử khoáng trong nghiên cứu có tiềm năng chế tạo mô xương ghép từ xương bò cho ứng dụng tái tạo mô xương trong nha khoa.

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