BACKGROUND: Infective endocarditis (IE) caused by non-HACEK gram-negative bacteria (nHGNB) is uncommon. In the 2023 Duke-ISCVID diagnostic criteria, METHODS: nHGNB IE cases reported to the Swedish Registry of Infective Endocarditis (SRIE) between 2008 and 2023 were identified. Episodes of bacteremia caused by nHGNB during the same period in Region Skåne were used as controls. Characteristics of IE caused by nHGNB were compared with those of other pathogens reported to the SRIE. RESULTS: One hundred fourteen episodes of nHGNB IE, of which 98 (87%) were definitive, were identified (1.5% of all cases). CONCLUSIONS: Our findings indicate that