Xây dựng hệ thống tái sinh cây dạ yến thảo kép đen (Petunia hybrida Hort.ẽ Vilm.-Andr.cv 'Midnight Gold) từ mô lá=Establishment of a High-Efficiency Regeneration System for Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’ from Leaf Tissues

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Tác giả: Thị Huyền Trang Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: KH Nông nghiệp VN, 2024

Mô tả vật lý: tr.1420-2428

Bộ sưu tập: Metadata

ID: 251269

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Black double petunia (Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’), belonging to the Solanacea family, is a high - value and popular potted ornamental plant on the flower market. This study aimed to establish an efficient in vitro regeneration system for Petunia hybrida “Midnight Gold”. A one-factor experiment was arranged in a completely randomized design with three replications. Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with various growth regulators and additives. The results showed that the optimal medium for shoot regeneration and multiplication was MS medium containing 1 mg/l benzyl adenine (BA) and 6.5 g/l agarose. For shoot development, MS medium with 30 g/l sucrose, 5% coconut water, and 6.5 g/l agarose was most effective for shoot tips, while 10% coconut water was preferred for nodal explants. Rooting was successfully induced on MS medium supplemented with 30 g/l sucrose, 0.1 g/l activated charcoal, and 6.5 g/l agarose. Acclimatized plantlets exhibited a 75% survival rate, reaching an average height of 7.83cm and producing 16.55 leaves per plant within four weeks. These findings provide a valuable protocol for Petunia hybrida “Midnight Gold” in vitro mass propagation, meeting the increasing market demand for this popular ornamental species.Dạ yến thảo kép đen (Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’), thuộc họ Cà (Solanacea), là cây hoa trồng chậu có giá trị cao và đang được ưa chuộng trên thị trường. Nghiên cứu nhân giống in vitro cây Dạ yến thảo kép đen từ mô lá nhằm đáp ứng nhu cầu cây giống Dạ yến thảo cho thị trường. Các thí nghiệm một nhân tố được bố trí theo khối hoàn toàn ngẫu nhiên với 3 lần nhắc lại. Kết quả cho thấy: môi trường MS bổ sung 1 mg/l BA, 30 g/l sucrose và 6,5 g/l agar thích hợp nhất để tái sinh chồi từ mô lá. Môi trường dưỡng chồi có nguồn gốc chồi ngọn phù hợp nhất là MS bổ sung 30 g/l sucrose, 5% nước dừa và 6,5 g/l agar. Môi trường dưỡng chồi có nguồn gốc đốt thân phù hợp nhất là MS bổ sung 30 g/l sucrose, 10% nước dừa và 6,5 g/l agar. Môi trường ra rễ thích hợp nhất là MS bổ sung 30 g/l sucrose, 0,1 g/l AC và 6,5 g/l agar. Cây con in vitro được trồng trên giá thể peatmoss thích nghi tốt sau 4 tuần ra ngôi với tỷ lệ sống đạt 75%, chiều cao cây đạt 7,83cm, số lá đạt 16,55 lá/cây.

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