Nghiên cứu biểu hiện gen OsHSBP1 liên quan tới đáp ứng chống chịu hạn và nóng ở lúa=Investigating OsHSBP1 Gene Expression in Relation to Drought and Heat Responses in Rice (Oryza sativa L.)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Lệ Quyên Cao

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: KH Nông nghiệp VN, 2024

Mô tả vật lý: tr.1429-1436

Bộ sưu tập: Metadata

ID: 251271

Thêm vào giỏ

Protein sốc nhiệt HSP (Heat Shock Protein) có vai trò quan trọng trong các phản ứng chống chịu stress liên quan tới nhiệt độ và oxy hóa, giúp bảo vệ tế bào và các đại phân tử khỏi các tác động bất lợi. Ở một số loài thực vật, quá trình điều hòa hoạt động của HSP có sự tham gia của protein HSBP (Heat Shock Factor Binding Protein). Để phục vụ nghiên cứu hoạt động chức năng và làm sáng tỏ cơ chế điều hòa đáp ứng stress của OsHSBP1 ở lúa, chúng tôi đã phân tích mô hình biểu hiện trong điều kiện stress hạn và nóng và trình tự của gen này ở hai giống lúa chủ lực BC15 và OM54541. OsHSBP1 tăng cường biểu hiện ở một số thời điểm của quá trình xử lý stress hạn và nóng nhân tạo. Trình tự mã hóa OsHSBP1 của hai giống lúa này đã được phân lập và giải trình tự nucleotide hoàn chỉnh. Đoạn gen phân lập được dài 237bp, mã hóa cho một chuỗi polypeptide dài 78 gốc axit amin, có chứa một chuỗi xoắn α, giống 59,8-97,4% so với các protein HSBP tương đồng đã được nghiên cứu. Kết quả này là tiền đề cho các nghiên cứu vai trò chức năng của OsHSBP1 và cải tiến khả năng chống chịu stress hạn và nóng của các chất lượng giống lúa phổ biến trong sản xuất như BC15 và OM5451 bằng công nghệ gen.The production of rice is significantly affected by adverse environmental conditions such as drought and heat. Heat shock proteins (HSPs) play a crucial role in mitigating stress tolerance responses associated with temperature and oxidation, thereby safeguarding cells and macromolecules from detrimental effects. The regulatory mechanism of HSP activity in some plant species involves the involvement of Heat Shock Factor Binding Protein (HSBP). This study aimed to investigate the functional activities and elucidate the stress response regulation mechanism of OsHSBP1 in rice. The expression pattern of this gene was analyzed under drought and heat stress conditions in two rice varieties, BC15 and OM54541. The OsHSBP1 expression increased at various time points during artificial heat and drought stress. The coding sequences of OsHSBP1 from these rice varieties were isolated and fully sequenced, revealing a gene segment of 237 base pairs in length. This segment encodes a polypeptide chain comprising 78 amino acids, including an α helix, exhibiting a similarity ranging from 59.8% to 97.4% homologous to HSBP proteins studied previously. These findings provide a foundation for exploring the functional role of OsHSBP1 to improve the drought and heat stress resistance of the major rice varieties such as BC15 and OM5451 through genetic engineering..

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR